Enhanced Protocol for Isolation of Plant Genomic DNA

Improved Protocol for Isolation of Genomic DNA from Leaf Tissues of Phyllanthus emblica Gaertn

Modified Cetyltrimethylammonium bromide (CTAB) protocol for DNA isolation was developed from leaf tissuesof Phyllanthus emblica for obtaining high quality genomic DNA. Fresh leaves of three different maturitywere analyzed for yield and quality of DNA. Acidity was determined in three different maturity of leaves viz.tender, intermediate and mature and their influence on DNA qua...

Modified CTAB protocol using a silica matrix for isolation of plant genomic DNA.

An Efficient Protocol for Genomic Dna Isolation from Cultivable Bacteria

A standardized protocol can play a major role in isolation of DNA especially when dealing with bacterial cultures. The aim of the present study was to develop a standard protocol of ultrapure high yield bacterial genomic DNA extraction from bacterial culture suitable for use in molecular biology. Extraction procedure was optimized with series of steps, which involved an unpublished modification...

A high-throughput, high-quality plant genomic DNA extraction protocol.

The isolation of high-quality genomic DNA (gDNA) is a crucial technique in plant molecular biology. The quality of gDNA determines the reliability of real-time polymerase chain reaction (PCR) analysis. In this paper, we reported a high-quality gDNA extraction protocol optimized for real-time PCR in a variety of plant species. Performed in a 96-well block, our protocol provides high throughput. ...

A standardized protocol for genomic DNA isolation from Terminalia arjuna for genetic diversity analysis